Sandwich ELISA:Article Title: Elucidation of the Immunotoxicity of PEDOT: PSS on RAW264.7 Macrophages by Oxidative Stress, Inflammatory Response, and NF-κB Pathway Activation
Article Snippet: Poly(3,4-ethylenedioxythiophene): poly(styrenesulfonate) (PEDOT: PSS) nanoparticles, widely used conductive polymers, pose environmental and health risks due to their nanoscale dispersion.. However, the characteristics of PEDOT: PSS in aquatic systems and the underlying mechanisms of its toxicity in animal and cell models remain poorly understood.. This study aimed to investigate the toxicological effects of PEDOT: PSS nanoparticles on macrophages, with a focus on RAW 264.7 cells.
Western Blot:Article Title: ZIKV envelope protein is a strong blocker of early directional differentiation in the neural lineage.
Article Snippet: The protein bands were visualized using the enhanced chemiluminescent (ECL) system (Perkin Elmer) and then analyzed using ChemoStar 6.0 Imaging Software (INTAS). .. Primary antibodies used in Western blotting include anti-DYKDDDDK-Tag-Mouse-Monoclonal (1:2000, Proteintech, Cat No. 66008-4-Ig), anti-PAX6-Rabbit-Polyclonal (1:1000, Proteintech, Cat No. 12323–1-AP), antiβ-III-Tubulin-Mouse-Monoclonal (1:2000, Abclonal, Cat No. A18132), anti-Cleaved Caspase3-Rabbit-Polyclonal (1:1000, Proteintech, Cat No. 25128-1-AP), anti- Caspase 9/P35-Rabbit-Polyclonal (1:300, Proteintech, Cat No. 10380-1-AP), anti-Phospho-P53(Ser15)-Rabbit-Polyclonal (1:2000, Proteintech, Cat No. 28961-1-AP), anti-BaxMouse-Polyclonal (1:10000, Proteintech, Cat No. 60283-2-Ig), anti-Bcl2-Rabbit-Polyclonal (1:500, Proteintech, AR TI CL E IN P RE SS Cat No. 26593-1-AP), anti-NLRP3-Rabbit-Polyclonal (1:1000, abcam, Cat No. ab4207), anti-GSDMD-RabbitMonoclonal (1:1000, abcam, Cat No. ab209845), anti-Caspase1-Rabbit-Monoclonal (1:1000, abcam, Cat No. ab207802), anti-KLF2-Rabbit-Polyclonal (1:200, Signalway Antibody, Cat No. #46591), anti-DPPA2-RabbitPolyclonal (1:1000, Signalway Antibody, Cat No. #62249), anti-Calretinin-Rabbit-Polyclonal (1:500, EpiZyme, Cat No. #P011307), anti-p57 Kip2-Rabbit-Monoclonal (1:500, Zenbio, Cat No. #R380868), anti-L1TD1-RabbitPolyclonal (1:1000, Signalway Antibody, Cat No. #62248), anti-WNT7A-Rabbit-Polyclonal (1:1000, Signalway Antibody, Cat No. #38653-1), anti-GRIK2-Rabbit-Monoclonal (1:500, Zenbio, Cat No. #R389225), anti-ROBO2Rabbit-Polyclonal (1:1000, Proteintech, Cat No. 21635-1-AP), anti-β-actin-Rabbit-Polyclonal (1:2000, Proteintech, Cat No: 20536-1-AP), anti-GAPDH-Mouse-Polyclonal (1:5000, EpiZyme, Cat No. LF205). .. The secondary antibodies include horseradish peroxidase (HRP)-conjugated Goat anti-Rabbit IgG (H + L) (1:2000, Abclonal, Cat No. A5014) or HRP-conjugated Goat anti-Mouse IgG (H + L) (1:5000, Proteintech, Cat No. SA0000-1-1).
Article Title: ZIKV envelope protein is a strong blocker of early directional differentiation in the neural lineage
Article Snippet: The protein bands were visualized using the enhanced chemiluminescent (ECL) system (Perkin Elmer) and then analyzed using ChemoStar 6.0 Imaging Software (INTAS). .. Primary antibodies used in Western blotting include anti-DYKDDDDK-Tag-Mouse-Monoclonal (1:2000, Proteintech, Cat No. 66008-4-Ig), anti-PAX6-Rabbit-Polyclonal (1:1000, Proteintech, Cat No. 12323–1-AP), anti-β-III-Tubulin-Mouse-Monoclonal (1:2000, Abclonal, Cat No. A18132), anti-Cleaved Caspase3-Rabbit-Polyclonal (1:1000, Proteintech, Cat No. 25128-1-AP), anti- Caspase 9/P35-Rabbit-Polyclonal (1:300, Proteintech, Cat No. 10380-1-AP), anti-Phospho-P53(Ser15)-Rabbit-Polyclonal (1:2000, Proteintech, Cat No. 28961-1-AP), anti-Bax-Mouse-Polyclonal (1:10000, Proteintech, Cat No. 60283-2-Ig), anti-Bcl2-Rabbit-Polyclonal (1:500, Proteintech, Cat No. 26593-1-AP), anti-NLRP3-Rabbit-Polyclonal (1:1000, abcam, Cat No. ab4207), anti-GSDMD-Rabbit-Monoclonal (1:1000, abcam, Cat No. ab209845), anti-Caspase1-Rabbit-Monoclonal (1:1000, abcam, Cat No. ab207802), anti-KLF2-Rabbit-Polyclonal (1:200, Signalway Antibody, Cat No. 46591), anti-DPPA2-Rabbit-Polyclonal (1:1000, Signalway Antibody, Cat No. 62249), anti-Calretinin-Rabbit-Polyclonal (1:500, EpiZyme, Cat No. P011307), anti-p57 Kip2-Rabbit-Monoclonal (1:500, Zenbio, Cat No. R380868), anti-L1TD1-Rabbit-Polyclonal (1:1000, Signalway Antibody, Cat No. 62248), anti-WNT7A-Rabbit-Polyclonal (1:1000, Signalway Antibody, Cat No. #38653-1), anti-GRIK2-Rabbit-Monoclonal (1:500, Zenbio, Cat No. R389225), anti-ROBO2-Rabbit-Polyclonal (1:1000, Proteintech, Cat No. 21635-1-AP), anti-β-actin-Rabbit-Polyclonal (1:2000, Proteintech, Cat No: 20536-1-AP), anti-GAPDH-Mouse-Polyclonal (1:5000, EpiZyme, Cat No. LF205). .. The secondary antibodies include horseradish peroxidase (HRP)-conjugated Goat anti-Rabbit IgG (H + L) (1:2000, Abclonal, Cat No. A5014) or HRP-conjugated Goat anti-Mouse IgG (H + L) (1:5000, Proteintech, Cat No. SA0000-1-1).
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